The research on centromere protein H promoting TSCC cells proliferation

doi:10.3877 / cma.j.issn.1674-1366.2009-04-003

Abstract

Abstract:

Objective

To investigate the expression pattern and promoting proliferation function of centromere protein H （CENP-H） in Squamous cell carcinoma of the tongue which has been demonstrated to be associated with aneuploidy in carcinoma cell.

Methods

Reverse transcriptase polymerase chain reaction （RT-PCR） and Western blot analysis were performed to test the expression of CENP-H in eight matched pairs of Squamous cell carcinoma of the tongue tissues （C） and adjacent noncancerous tongue tissues （N）， as well as Squamous cell carcinoma of the tongue （TSCC） cell lines and tongue epithelial cells （TEC）， the level of CENP-H in 8 characterized TSCC tissues was determined by immunohistochemistry. MTT assay， colony formation assay displayed that TSCCa with high expression of CENP-H reproductive activity.

Results

The mRNA and protein level of CENP-H was much higher in cancer tissues （C） and TSCC cell lines than that in adjacent noncancerous tongue tissues （N） and in normal cells， respectively. MTT assay， colony formation assay displayed that high expression of CENP-H promoted the proliferation of TSCC cell line TSCCa （P ＜0.001）.

Conclusion

CENP-H is highly expressed in TSCC， and its high expression promotes proliferation.

Key words: Squamous cell carcinoma of the tongue, CENP-H, Proliferation

Dong-hui WU, Jin-song LI, Zhao-yu LIN, Wei-liang CHEN, Chao-bin PAN, Wen-ting LIAO, Chun-ping YU, Qing-li KONG, Li-bing SONG. The research on centromere protein H promoting TSCC cells proliferation[J]. Chinese Journal of Stomatological Research(Electronic Edition), 2009, 3(04): 361-368.

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URL: https://zhkqyxyjzz.cma-cmc.com.cn/EN/10.3877 / cma.j.issn.1674-1366.2009-04-003

https://zhkqyxyjzz.cma-cmc.com.cn/EN/Y2009/V3/I04/361

Figures/Tables 9

References 10

1	Amor DJ， Kalitsis P， Sumer H， et al. Building the centromere： from foundation proteins to 3D organization. Trends Cell Biol， 2004，14（7）：359-368.
2	Orthaus S， Ohndorf S， Diekmann S. RNAi knockdown of human kinetochore protein CENP-H. Biochem Biophys Res Commun， 2006，348（1）：36-46.
3	Tomonaga T， Matsushita K， Ishibashi M， et al. Centromere protein H is up-regulated in primary human colorectal cancer and its overexpression induces aneuploidy. Cancer Res， 2005，65（11）：4683-4689.
4	Shigeishi H， Higashikawa K， Ono S， et al. Increased expression of CENP-H gene in human oral squamous cell carcinomas harboring highproliferative activity. Oncol Rep， 2006，16（5）：1071-1075.
5	Liao WT， Song LB， Zhang HZ， et al. Centromere protein H is a novel prognostic marker for nasopharyngeal carcinoma progression and overall patient survival. Clin Cancer Res， 2007，13（2 Pt 1）：508-514.
6	Weaver BA， Cleveland DW. Aneuploidy：instigator and inhibitor of tumorigenesis. Cancer Res， 2007，67（21）：10103-10105.
7	Doak SH. Aneuploidy in upper gastro-intestinal tract cancers—a potential prognostic marker？Mutat Res，2008，651（1-2）：93-104.
8	Sugata N， Munekata NSE， Todokoro K. Characterization of a novel kinetochore protein， CENP-H. J Biol Chem， 1999，274（39）：27343-27346.
9	Sugata N， Li S， Earnshaw WC， et al. Human CENP-H multimers colocalize with CENP-A and CENP-C at active centromere-kinetochore complexes. Hum Mol Genet， 2000，9（19）：2919-2926.
10	Neville BW，Day TA. Oral cancer and precancerous lesions. CA Cancer J Clin， 2002，52（4）：195-215.

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